Emily Sevcik, John Szymanksi and Quentin Jallerat are co-authors on our recent article in Current Protocols in Cell Biology entitled “Patterning on Topography for Generation of Cell Culture Substrates with Independent Nanoscale Control of Chemical and Topographical Extracellular Matrix Cues.” In the article we provide detailed methods on how to perform our Patterning on Topography (PoT) technology to independently pattern extracellular matrix proteins on topographically complex surfaces for studying cell-material interactions.