The Regenerative Biomaterials and Therapeutics Group is located at the Pittsburgh Technology Center and operates a biomaterials and cell biology research lab as well as administering a number of shared-use instruments for the Biomedical Engineering Department and the Carnegie Institute of Technology.
Major Equipment
- Asylum Research MFP-3D-BIO Atomic Force Microscope (AFM)
- Zeiss LSM 700 Laser Scanning Confocal Microscope
- Nikon AZ100-C2 Laser Scanning Confocal Microscope
- Nikon SMZ1500 Epifluorescent Stereomicroscope
- Nikon A1R HD Confocal and Multiphoton Microscope
- Leica TP5 Multiphoton Microscope
- Nikon Ti-E Deconvolution Microscope
- Nikon TS100F Epifluorescent Inverted Microscope
- Advanced Image Processing Workstation with Imaris (9.1) and AutoQuant Deconvolution Software
- BD FACSJazz Fluorescently Activated Cell Sorter
- Thorlabs VEGA VEG210C1 Optical Coherence Tomography System (OCT)
- 3D Printing Lab
Additional Resources
- Cell Culture Facilities
- Polymer Processing/Fabrication
- Rapid Prototyping and 3D Printing
- Mechanical Testing Instruments
- MTS Acumen Mechanical Testing System
- Instron 5900 Desktop Mechanical Testing Instrument
Atomic Force Microscope
Asylum Research MFP-3d-BIO Atomic Force Microscope with Integrated Nikon Ti Inverted Fluorescent Microscope
The AFM is integrated with a Nikon Ti inverted epifluorescent microscope for coupled optical and AFM imaging of biological samples. The Nikon Ti is equipped with 10x, 40x and 60x objectives, filter cubes for DAPI, FITC and TRITC and a Hamamatsu ORCA-Flash 2.8 Scientific CMOS camera.
This equipment is maintained by the Regenerative Biomaterials Group, but is a shared resource and is part of the Center for the Mechanics and Engineering of Cellular Systems. Interested CMU students and faculty can gain access to this system after training.
New users must follow the training protocol.
Confocal Microscope Imaging Systems
Zeiss LSM 700 Laser Scanning Confocal Microscope
Fully motorized inverted microscope with full incubation and 4 laser lines.
Nikon AZ100 C2 Laser Scanning Confocal Microscope
Multizoom 8x macroscope with 1x and 5x objectives coupled to a C2 laser scanning confocal microscope (4 laser lines) for imaging embryos, tissue engineered constructs and other millimeter to microscale structures.
This equipment is a shared resource, interested CMU students and faculty should contact Prof. Feinberg for access.
Multiphoton Microscope Imaging Systems
Nikon A1R HD Confocal and Multiphoton Microscope
Fully automated Nikon upright microscope equipped with 4 laser lines, one tunable multiphoton line, and another fixed 1300 nm line. The system has a high speed piezo z-stage and an ultra-fast resonant scanner with 4 high sensitivity non-descanned detectors and 4 additional descanned detectors. The system has several extra-long working distance high numerical aperture objectives, including objectives that can be dipped into multiple clearing solutions for imaging thick tissues.
Leica TP5 Multiphoton Microscope
A fully automated Leica DMI6000B inverted microscope, equipped with four laser lines and a laser scanning confocal scan head as well as a tunable
Ti:Sapphire lased and hybrid detectors for multiphoton imaging. The system is enclosed in an incubator providing a heated, humidified and gassed environment for on-stage cell and engineered tissue culture to support live cell imaging and includes focus control to maintain focus over prolonged imaging times.
Additional Optical Imaging Systems
Nikon TS100F Inverted Epifluorescence Microscope
Standard inverted epifluorescence microscope equipped with 5x, 10x, 20x and 40x objectives and DAPI, FITC and TRITC filter cubes. Digital capture using a Photometrics CoolSnap ES or Cascade 1K CCD camera.
Nikon Ti-E Deconvolution Microscope
Nikon SMZ 1000 Stereomicroscope
Leica
3D Printing Lab
Cell Culture Facilities
Polymer Processing/Fabrication
