Fibronectin-Based Nanomechanical Biosensors to Map 3D Strains in Live Cells and Tissue

Dan Shiwarski, Josh Tashman, Alkiviadis Tsamis, Jaci Bliley, Malachi Blundon, Edgar Aranda-Michel, Quentin Jallerat, John Szymanski, Brooke McCartney and Adam Feinberg are co-authors on our recent article in Nature Communications entitled “Fibronectin-Based Nanomechanical Biosensors to Map 3D Strains in Live Cells and Tissues.” The ability to measure strain in cells and tissues in vitro with minimal perturbation and at high spatial resolution has proven challenging. Here, we have developed a fluorescently-labelled nanomechanical biosensor (NMBS) made of a fibronectin square lattice mesh with tunable resolution that can be applied to the surface of cells and tissues to enable direct quantification and mapping of strain over time. Additionally, we have released an open-source MATLAB and Imaris biomechanics software package to map and quantify 3D surface strain, cardiomyocyte beat frequency, and area dilation from live fluorescence imaging data. Accompanying the manuscript is a published protocol detailing the step-by-step NMBS fabrication process.

Read the article at Nature Communications

FRESH 3D Bioprinting a Full-Size Model of the Human Heart

Eman Mirdamadi, Josh Tashman, Dan Shiwarski, Rachelle Palchesko, and Adam Feinberg are co-authors on our recent article in ACS Biomaterials Science & Engineering entitled “FRESH 3D Bioprinting a Full-Size Model of the Human Heart.” In the article, we demonstrate the FRESH platform’s capabilities in printing acellular, full-sized human heart models from alginate, which possesses similar mechanical properties to cardiac tissue, enabling surgeons to one day use our models for realistic surgical simulation.

Read the article at ACS Biomaterials Science & Engineering

Congratulations to Undergraduate Researcher Sanjana Shah for receiving the Andrew Carnegie Scholarship

ACS Scholars are undergraduate seniors who embody the University’s high standards of academic excellence, volunteerism, leadership and involvement in student organizations, athletics or the arts. The winners of the ACS Scholars program are carefully selected each year by deans/department heads from across Carnegie Mellon. Congratulations Sanjana, click here to read more!

Congratulations to Emily Sevcik for Receiving Her PhD

Congratulations to Dr. Emily Sevcik for receiving her PhD in Biomedical Engineering. Her dissertation is titled “Understanding Fibronectin Mechanobiology of Growth Factor Binding Domain Availability and Cellular Extracellular Matrix Remodeling.” Her research has been published in Current Protocols in Cell Biology and Biomaterials Science. Emily was a pivotal member of the lab and will be greatly missed!

Congratulations to undergraduate Xining Gao for receiving the NSF GRFP!

Congratulations to Xining Gao, an undergraduate researcher in the RBG group, who has received the prestigious National Science Foundation (NSF) Graduate Research Fellowship Program (GFRP). Xining is a dual major in Biomedical Engineering (BME) and Material Science Engineering (MSE), who will graduate in May 2020, and plans to continue her education after graduation as a PhD student in BME. We are so excited to see what she will accomplish!

Feinberg Lab is awarded a grant from the DARPA BETR project involving “smart” wound healing

The Feinberg Lab is awarded a grant from the Defense Advanced Research Projects Agency’s (DARPA) Bioelectronics for Tissue Regeneration (BETR) project for the repair of volumetric muscle loss. The Feinberg lab will work in collaboration with labs from Carnegie Mellon University, University of Pittsburgh, Northwestern University, the University of Vermont, the University of Wisconsin and Walter Reed National Military Medical Center to generate the Regenerative Electronics Platform through Advanced Intelligent Regulation (REPAIR) patch, designed to help regrow fully functional muscle tissue. Click here to read more about this project.

Source: Jonathan Rivney

Congratulations to Andrew Lee for Receiving his PhD

Congratulations to Dr. Andrew Lee for receiving his PhD in Biomedical Engineering. His dissertation is titled “3D Bioprinting of Collagen to Engineer Muscle Tissues with Complex Architectures.” Andrew has received a number of awards and recognitions as a PhD student in the group. His research has been published in Science and ACS Biomaterials Science & Engineering. Andrew is continuing his career as the Head of Bioprinting at FluidForm.