{"id":1599,"date":"2020-11-18T09:11:00","date_gmt":"2020-11-18T16:11:00","guid":{"rendered":"https:\/\/regenerativebiomaterials.com\/?p=1599"},"modified":"2021-10-20T13:22:27","modified_gmt":"2021-10-20T20:22:27","slug":"fibronectin-based-nanomechanical-biosensors-to-map-3d-strains-in-live-cells-and-tissue","status":"publish","type":"post","link":"https:\/\/regenerativebiomaterials.com\/wordpress\/?p=1599","title":{"rendered":"Fibronectin-Based Nanomechanical Biosensors to Map 3D Strains in Live Cells and Tissue"},"content":{"rendered":"\n<p>Dan Shiwarski, Josh Tashman, Alkiviadis Tsamis, Jaci Bliley, Malachi Blundon, Edgar Aranda-Michel, Quentin Jallerat, John Szymanski, Brooke McCartney and Adam Feinberg are co-authors on our recent article in <strong><em>Nature Communications<\/em><\/strong> entitled \u201cFibronectin-Based Nanomechanical Biosensors to Map 3D Strains in Live Cells and Tissues.\u201d The ability to measure strain in cells and tissues in vitro with minimal perturbation and at high spatial resolution has proven challenging. <a rel=\"noreferrer noopener\" href=\"https:\/\/zenodo.org\/record\/4064033#.X7vTRctKhro\" target=\"_blank\">Here<\/a>, we have developed a fluorescently-labelled nanomechanical biosensor (NMBS) made of a fibronectin square lattice mesh with tunable resolution that can be applied to the surface of cells and tissues to enable direct quantification and mapping of strain over time. Additionally, we have released an open-source MATLAB and Imaris biomechanics <a rel=\"noreferrer noopener\" href=\"https:\/\/zenodo.org\/record\/4065743#.X7vTLctKhro\" target=\"_blank\">software package<\/a> to map and quantify 3D surface strain, cardiomyocyte beat frequency, and area dilation from live fluorescence imaging data. Accompanying the manuscript is a published <a rel=\"noreferrer noopener\" href=\"https:\/\/doi.org\/10.21203\/rs.3.pex-1169\/v1\" target=\"_blank\">protocol<\/a> detailing the step-by-step NMBS fabrication process.<\/p>\n\n\n\n<p><a rel=\"noreferrer noopener\" href=\"https:\/\/doi.org\/10.1038\/s41467-020-19659-z\" target=\"_blank\"><strong>Read the article at Nature Communications<\/strong><\/a><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/regenerativebiomaterials.com\/wordpress\/wp-content\/uploads\/2020\/05\/DanPicture-1.jpeg\" alt=\"\" class=\"wp-image-1216\" width=\"407\" height=\"498\" srcset=\"https:\/\/regenerativebiomaterials.com\/wordpress\/wp-content\/uploads\/2020\/05\/DanPicture-1.jpeg 543w, https:\/\/regenerativebiomaterials.com\/wordpress\/wp-content\/uploads\/2020\/05\/DanPicture-1-245x300.jpeg 245w\" sizes=\"(max-width: 407px) 100vw, 407px\" \/><\/figure><\/div>\n\n\n\n<hr class=\"wp-block-separator is-style-wide\"\/>\n","protected":false},"excerpt":{"rendered":"<p>Dan Shiwarski, Josh Tashman, Alkiviadis Tsamis, Jaci Bliley, Malachi Blundon, Edgar Aranda-Michel, Quentin Jallerat, John Szymanski, Brooke McCartney and Adam Feinberg are co-authors on our recent article in Nature Communications entitled \u201cFibronectin-Based Nanomechanical Biosensors to Map 3D Strains in Live Cells and Tissues.\u201d The ability to measure strain in cells and tissues in vitro with &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/regenerativebiomaterials.com\/wordpress\/?p=1599\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Fibronectin-Based Nanomechanical Biosensors to Map 3D Strains in Live Cells and Tissue&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3,4,5],"tags":[],"_links":{"self":[{"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1599"}],"collection":[{"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1599"}],"version-history":[{"count":4,"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1599\/revisions"}],"predecessor-version":[{"id":1746,"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1599\/revisions\/1746"}],"wp:attachment":[{"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1599"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1599"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/regenerativebiomaterials.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1599"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}